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J.V. Neves et al.
starting earlier and reaching higher levels in Hamp-/-, where ferroportin could already be observed at day 7, with much higher levels at day 21 (Figure 7B). No signif- icant changes in ferroportin protein levels were observed in the duodenum (Figure 7C).
Discussion
Using a mouse model of trypanosomiasis (which has been shown to replicate the two stages of the disease31 occurring in humans) we performed an integrated analysis
A
B
C
Figure 7. Ferroportin pro- tein levels in the liver, spleen and duodenum of C57BL/6 and Hamp-/- mice during infection T.b. brucei, analyzed by Western blot. (A) Liver. (B) Spleen. (C) Duodenum. GAPDH was used as housekeeping protein. Graphs represent the den- sitometry analysis of FPN1 protein in each day post infection, normalized to GAPDH densitometry, expressed as percentage to non-infected C57BL/6 animals. Values are repre- sented as mean±standard deviation (n=4). Differences among groups were considered signifi- cant at P<0.05, P<0.01, and P<0.001, represented respectively by the letters a, b, c between control and infected C57BL/6 mice, d, e, f between con- trol and infected Hamp-/- mice and g, h, i between C57BL/6 control and Hamp-/- mice. NI: non- infected; dpi: days post infection.
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haematologica | 2021; 106(3)