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S. Yang et al.
Considering that there were few adipocytes in the BM of C57BL/6 mice, we raised the mice with a high-fat diet to increase the number of adipocytes in the BM. BM adipocytes in obese mice are round or elliptical in shape
(Figure 5A). Immunohistochemical staining of perilipin1 protein in the BM adipocytes showed that the BM adipocytes stained yellow (Figure 5A). According to the pathogenic characteristics of FBL-3 cells,29 BM samples
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Figure 5. TRPV4 plays an important role in bone adipocyte remodeling in acute myeloid leukemia mice. (A) Hematoxylin & eosin staining and immunohistochemical staining with perilipin1 antibody in bone marrow (BM) sections from the controls (Ctr, n=5) and experimental mice, (mice with acute myeloid leukemia [AML], n=5; AML mice treated with 4a-phorbol 12,13-didecanoate [4aPDD], n=5). Ten fields were analyzed for each mouse at 400× magnification. Three independent experi- ments were performed. Scale bars represent 50 mm and 100 mm, respectively. (B, C) Adipocyte number and area in the controls, AML mice and AML mice treated with 4aPDD, quantitatively analyzed by Image-Pro-Plus 5.1. (D) Immunofluorescence was used to analyze the expression of CD117 in BM sections of the controls, AML mice and AML mice treated with 4aPDD. 4′,6-diamidino-2-phenylindole (DAPI) stained blue and CD117 showed red fluorescence. Scale bars represent 100 mm. (E) The GDF15 content of the BM supernatant in the controls and AML mice was analyzed by enzyme-linked immunosorbent assay. (F) Kaplan-Meier curves showing the overall survival rate of AML mice (n=9) and AML mice treated with 4aPDD (n=9). Three independent experiments were performed. ***P<0.001, **P<0.01, *P<0.05.
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haematologica | 2020; 105(11)