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haematologica | 2020; 105(11)
Transfusion Medicine
Genetic platelet depletion is superior in platelet transfusion compared to current models
Manuel Salzmann,1 Waltraud C. Schrottmaier,1 Julia B. Kral-Pointner,1 Marion Mussbacher,1 Julia Volz,3 Bastian Hoesel,1 Bernhard Moser,1 Sonja Bleichert,1,2 Susanne Morava,1 Bernhard Nieswandt,3 Johannes A. Schmid1 and Alice Assinger1
1Institute of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria, 2Department of Surgery, General Hospital, Medical University Vienna, Vienna, Austria and 3Institute of Experimental Biomedicine, University Hospital and Rudolf Virchow Center, University of Würzburg, Würzburg, Germany
doi:10.3324/haematol.2020.266072 ©2020 Ferrata Storti Foundation
An incorrect version of Figure 5 appeared in the June 2020 issue on page 1744. The correct version of Figure 5 is published on this page.
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Figure 5. Platelet transfusion efficacy and donor platelet function analysis of iDTRPlt mice. (A) Graphical overview for comparison of platelet transfusion. DT treatment started 7 days prior to transfusion and R300 treatment 12 hours prior to transfusion. Blood was taken at basal and depleted state, and 2, 24, 48, and 72 hours after transfusion. (B) Percentage of total, transfused, and endogenous platelet counts, relative to initial counts. Transfused platelets were labeled with an anti-GPIbβ-Dylight649 antibody. Theoretical, maximal transfusion is depicted as grey area. n = 5. (C) Graphical overview of donor platelet function evaluation. DT treatment started 7 days prior to transfusion and blood was taken at basal and depleted state, and 24 h after transfusion. (D) Comparison of percentage of CD62P+ and activated GPIIb/IIIa+ platelets in whole blood, freshly drawn from donors and after circulating for 24 hours in iDTRPlt mice. n = 4-9. (E) Concentration of plasma CXCL4 of iDTRPlt mice at basal and depleted levels, and 24 h after platelet transfusion. n = 5 (F) Concentration of circulating exogenous platelets after transfusion of indicated numbers of platelets. n = 5-10.
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