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ADAMTS13 variants to escape autoantibodies in iTTP
Figure 2. Heat map with reactivity of all the patients’ samples tested against all ADAMTS13 spacer exosite-3 RFRYY mutant variants created for this study. Mutants (left) are organized by mutation type with residues mutated in color and underlined (blue: conservative mutations; green: semi-conservative mutations; red: non-con- servative mutations; gray: alanine mutations; purple: arginine-to-lysine mutations), and by number of mutations introduced. The classic gain-of-function (GoF) variant is displayed separately for comparison. Patients’ dilution factors and antibody titers are indicated in the rows below. The median reactivity of each mutant is displayed in the same heat-map fashion (right column). A color scale for reactivity is displayed below with green backgrounds indicating low reactivity, white backgrounds inter- mediate reactivity and red backgrounds high reactivity (n=2).
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