Page 74 - Haematologica-5

806
M. Schneeweiss et al.
A
B
C
Figures 5. Effects of DCC-2618 and DP-5439 on proliferation and survival of acute myeloid leukemia (AML) and chronic myelomonocytic leukemia (CMML). (A,C) MOLM-13, MV4-11, KG-1, U937 and primary leukemic cells were incubated in control medium (0 μM) or medium containing various concentrations of DCC-2618 or DP-5439, as indicated at 37°C for 48 h. Thereafter, 3H-thymidine uptake was determined. Results in (A) are expressed as percent of control and represent the mean±S.D. from three independent experiments. Asterisk (*): P<0.05 compared to control medium. Results of (C) are expressed as percent of control and represent the mean±S.D. from triplicates. (B) MOLM-13, MV4-11, KG-1 and U937 cells were incubated with control medium (0 nM) or various concentrations of DCC-2618 and DP-5439, as indicated, for 48 h. Thereafter cells were harvested and the percentage of apoptotic cells was quantified morphologically on Wright-Giemsa-stained cytospin preparations (left panels) or by flow cytometry (determination of annexinV/PI-positive cells, right panels). Results represent the mean±S.D. of three inde- pendent experiments. Asterisk (*): P<0.05 compared to control medium.
haematologica | 2018; 103(5)