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J. Köhler et al.
A
B
to physiological hemostasis, and activation occurs in vivo always under pathological conditions, such as thrombosis, sepsis, or ARDS.40-42 This is the first study that investigated the functional contribution of PK in host defense to bacte- rial sepsis in rodent and human systems. The study shows that FXII and PK play distinct roles within the infection process. PK supports streptococcal spreading by its profib- rinolytic function, whereas, in our model, FXII did not influence bacterial dissemination.
Acknowledgments
We thank Jana Bull (IMIKRO) and Dr. Armin Springer
Figure 7. Significant decrease in plasma prekallikrein and factor XII (FXII) in plasma of patients with sepsis, severe sepsis or septic shock. Blood samples were collected from patients diagnosed with sepsis. Specific sandwich ELISA was performed to detect the levels of (A) plasma kallikrein (PK) and (B) FXII in plasma of sepsis patients on days 1-3. Non-survivor: n=8; survivor: n=15; healthy controls: n=15. ***P≤0.001.
(EMZ) for excellent technical assistance and Dr. Ulf Broschewitz for assessment of histology. We are also grateful to Ionis Pharmaceutical for providing us with ASO.
Funding
This study was supported by a grant from the Deutsche Forschungsgemeinschaft (project OE 547/ 4-1). SOH was sup- ported by the Federal Excellence Initiative of Mecklenburg Western Pomerania and European Social Fund (ESF) Grant KoInfekt (ESF_14-BM-A55-00xx_16) and by a grant from the Medical Faculty of the University of Rostock in the framework of the FORUN program 2018.
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