Page 148 - Haematologica April 2020

L. Boutin et al.
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Figure 2. Co-culture between acute myeloid leukemia (AML) blasts and mesenchymal stromal cells (MSC) modulates the ABC transporter functionality on leukemia blasts in a patient-dependent manner. (A-C) Percentage of AML blasts that efflux specific probes (ABC transporter activity) after a 3-day co-culture on healthy donor (HD) MSC. ABC transporter activities were quantified using specific probes (Dioc2,3, Purpurin 18, CMFDA for ABCB1, ABCG2, ABCC1, respectively) as shown on the histograms. ABCB1 activity is significantly increased (P=0.0059, n=9, Wilcoxon test) by AML blasts after a 3-day co-culture on HD MSC. (D-F) Corresponding probe mean fluorescence intensity (MFI) in AML blasts; Dioc2,3 MFI is significantly decreased (P=0.02, n=9, Wilcoxon test) in blasts after co-culture with HD MSC. (G-I) Percentage of AML blasts that efflux specific probes after a 3-day co-culture on AML MSC. Activity of the ABCB1, ABCG2 and CMFDA transporters was significantly increased (P=0.01, P=0.02 and P=0.008, respectively; n=3 without MSC and n=9 with AML MSC, Wilcoxon test). (J-L) Corresponding MFI in AML blasts. Purpurin 18 and CMFDA MFI are significantly decreased (P=0.01 and P=0.01, respectively; n=3 without MSC and n=9 with AML MSC, Wilcoxon test) in co- culture conditions. *P<0.05; **P<0.01.
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haematologica | 2020; 105(4)