Page 107 - Haematologica April 2020
P. 107

GPX4 controls reticulocyte maturation
Funding
MUM acknowledges funding from the Deutsche Forschungsgemeinschaft (SFB1036, SFB1118); SA is supported by the European Hematology Association (Advanced Research fellowship). Support by the German Federal Ministry of Education and Research Infrafrontier grant 01KX1012 for German Mouse Clinic (GMC) infrastructure (to MHA). MK and KO were funded by a grant from SNF 310030_163443/1.
Acknowledgments
We thank Prof. Anton Berns, the Netherland Cancer Institute, Amsterdam, for providing Rosa26-CreERT2 mice. MC and GWB thank Matilde Maiorino and Fulvio Ursini for many helpful discussions. We are most grateful to Martina Mötscher, Jasmin Teutsch and Michael Hagemann for breeding the mice.
References
1. Ursini F, Maiorino M, Valente M, Ferri L, Gregolin C. Purification from pig liver of a protein which protects liposomes and bio- membranes from peroxidative degradation and exhibits glutathione peroxidase activi- ty on phosphatidylcholine hydroperoxides. Biochim Biophys Acta. 1982;710(2):197- 211.
2. Maiorino M, Conrad M, Ursini F. GPx4, Lipid Peroxidation, and Cell Death: Discoveries, Rediscoveries, and Open Issues. Antioxid Redox Signal. 2018; 29(1):61-74.
3. Hentze MW, Muckenthaler MU, Andrews NC. Balancing acts: molecular control of mammalian iron metabolism. Cell. 2004; 117(3):285-297.
4. Bordet JC, Guichardant M, Lagarde M. Hydroperoxides produced by n-6 lipoxy- genation of arachidonic and linoleic acids potentiate synthesis of prostacyclin related compounds. Biochim Biophys Acta. 1988; 958(3):460-468.
5. Seiler A, Schneider M, Forster H, et al. Glutathione peroxidase 4 senses and trans- lates oxidative stress into 12/15-lipoxyge- nase dependent- and AIF-mediated cell death. Cell Metab. 2008;8(3):237-248.
6. Thomas JP, Maiorino M, Ursini F, Girotti AW. Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxi- dation. In situ reduction of phospholipid and cholesterol hydroperoxides. J Biol Chem. 1990;265(1):454-461.
7. Weitzel F, Wendel A. Selenoenzymes regu- late the activity of leukocyte 5-lipoxyge- nase via the peroxide tone. J Biol Chem. 1993;268(9):6288-6292.
8. Schnurr K, Belkner J, Ursini F, Schewe T, Kuhn H. The selenoenzyme phospholipid hydroperoxide glutathione peroxidase con- trols the activity of the 15-lipoxygenase with complex substrates and preserves the specificity of the oxygenation products. J Biol Chem. 1996;271(9):4653-4658.
9. Kuhn H, Borchert A. Regulation of enzy- matic lipid peroxidation: the interplay of peroxidizing and peroxide reducing enzymes. Free Radic Biol Med. 2002; 33(2):154-172.
10. Kryukov GV, Castellano S, Novoselov SV, et al. Characterization of mammalian selenoproteomes. Science. 2003; 300(5624):1439-1443.
11. Burk RF, Hill KE. Regulation of selenium metabolism and transport. Annu Rev Nutr. 2015;35:109-134.
12. Ingold I, Berndt C, Schmitt S, et al. Selenium Utilization by GPX4 is required to orevent hydroperoxide-induced ferrop- tosis. Cell. 2018;172(3):409-422.
13. Liao C, Hardison RC, Kennett MJ, Carlson BA, Paulson RF, Prabhu KS. Selenoproteins regulate stress erythroid progenitors and spleen microenvironment during stress ery- thropoiesis. Blood. 2018;131(23):2568- 2580.
14. Kaushal N, Hegde S, Lumadue J, Paulson RF, Prabhu KS. The regulation of erythro- poiesis by selenium in mice. Antioxid Redox Signal. 2011;14(8):1403-1412.
15. Schweers RL, Zhang J, Randall MS, et al. NIX is required for programmed mitochon- drial clearance during reticulocyte matura- tion. Proc Natl Acad Sci U S A. 2007; 104(49):19500-19505.
16. Sandoval H, Thiagarajan P, Dasgupta SK, et al. Essential role for Nix in autophagic mat- uration of erythroid cells. Nature. 2008; 454(7201):232-235.
17. Chu CT, Ji J, Dagda RK, et al. Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells. Nat Cell Biol. 2013;15(10):1197-1205.
18. Kagan VE, Tyurina YY, Tyurin VA, et al. Cardiolipin signaling mechanisms: collapse of asymmetry and oxidation. Antioxid Redox Signal. 2015;22(18):1667-1680.
19. Morgan AH, Hammond VJ, Sakoh- Nakatogawa M, et al. A novel role for 12/15-lipoxygenase in regulating autophagy. Redox Biol. 2015;4:40-47.
20. Schewe T, Halangk W, Hiebsch C, Rapoport SM. A lipoxygenase in rabbit reticulocytes which attacks phospholipids and intact mitochondria. FEBS Lett. 1975;60(1):149-152.
21. Rapoport SM, Schewe T, Wiesner R, et al. The lipoxygenase of reticulocytes. Purification, characterization and biological dynamics of the lipoxygenase; its identity with the respiratory inhibitors of the retic- ulocyte. Eur J Biochem. 1979;96(3):545-561.
22. Rapoport SM, Schewe T. The maturational breakdown of mitochondria in reticulo- cytes. Biochim Biophys Acta. 1986;864(3- 4):471-495.
23. Kuhn H, Brash AR. Occurrence of lipoxyge- nase products in membranes of rabbit retic- ulocytes. Evidence for a role of the reticulo- cyte lipoxygenase in the maturation of red cells. J Biol Chem. 1990;265(3):1454-1458.
24. van Leyen K, Duvoisin RM, Engelhardt H, Wiedmann M. A function for lipoxygenase in programmed organelle degradation. Nature. 1998;395(6700):392-395.
25. Grullich C, Duvoisin RM, Wiedmann M, van Leyen K. Inhibition of 15-lipoxygenase leads to delayed organelle degradation in the reticulocyte. FEBS Lett. 2001;489(1):51-54.
26. Sun D, Funk CD. Disruption of 12/15- lipoxygenase expression in peritoneal macrophages. Enhanced utilization of the 5-lipoxygenase pathway and diminished oxidation of low density lipoprotein. J Biol
Chem. 1996;271(39):24055-24062.
27. Szebeni J, Winterbourn CC, Carrell RW. Oxidative interactions between haemoglo- bin and membrane lipid. A liposome
model. Biochem J. 1984;220(3):685-692. 28. NaveenKumar SK, SharathBabu BN, Hemshekhar M, Kemparaju K, Girish KS, Mugesh G. The role of reactive oxygen species and ferroptosis in heme-mediated activation of human platelets. ACS Chem
Biol. 2018;13(8):1996-2002.
29. Shah R, Shchepinov MS, Pratt DA.
Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent Sci. 2018;4(3):387-396.
30. Yant LJ, Ran Q, Rao L, et al. The selenopro- tein GPX4 is essential for mouse develop- ment and protects from radiation and oxidative damage insults. Free Radic Biol Med. 2003;34(4):496-502.
31. Friedmann Angeli JP, Schneider M, Proneth B, et al. Inactivation of the ferroptosis regu- lator Gpx4 triggers acute renal failure in mice. Nat Cell Biol. 2014;16(12):1180-1191.
32. Hameyer D, Loonstra A, Eshkind L, et al. Toxicity of ligand-dependent Cre recombi- nases and generation of a conditional Cre deleter mouse allowing mosaic recombina- tion in peripheral tissues. Physiol Genomics. 2007;31(1):32-41.
33. Higashi AY, Ikawa T, Muramatsu M, et al. Direct hematological toxicity and illegiti- mate chromosomal recombination caused by the systemic activation of CreERT2. J Immunol. 2009;182(9):5633-5640.
34. Loonstra A, Vooijs M, Beverloo HB, et al. Growth inhibition and DNA damage induced by Cre recombinase in mam- malian cells. Proc Natl Acad Sci U S A. 2001;98(16):9209-9214.
35. Zhu J, Nguyen MT, Nakamura E, Yang J, Mackem S. Cre-mediated recombination can induce apoptosis in vivo by activating the p53 DNA damage-induced pathway. Genesis. 2012;50(2):102-111.
36. Janbandhu VC, Moik D, Fassler R. Cre recombinase induces DNA damage and tetraploidy in the absence of loxP sites. Cell Cycle. 2014;13(3):462-470.
37. Pepin G, Ferrand J, Honing K, et al. Cre- dependent DNA recombination activates a STING-dependent innate immune response. Nucleic Acids Res. 2016;44(11): 5356-5364.
38. Huh WJ, Khurana SS, Geahlen JH, Kohli K, Waller RA, Mills JC. Tamoxifen induces rapid, reversible atrophy, and metaplasia in mouse stomach. Gastroenterology. 2012; 142(1):21-24.
39. Velasco-Hernandez T, Sawen P, Bryder D, Cammenga J. Potential pitfalls of the Mx1- Cre system: implications for experimental modeling of normal and malignant Hematopoiesis. Stem Cell Reports. 2016; 7(1):11-18.
haematologica | 2020; 105(4)
949


































































































   105   106   107   108   109