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KIT D816V dPCR in FFPE Sections
AB
CD
Figure 2. Association of KIT D816V allele burden with biomarkers of dis- ease burden in systemic mastocytosis (SM). Correlation of KIT D816V muta- tion burden in 185 formalin-fixed paraf- fin-embedded (FFPE) bone marrow (BM) sections [variant allele frequency (VAF) tissue: A and C] and 108 BM aspirate/peripheral blood (PB) samples (VAF liquid: B and D) with immunohisto- logically determined BM mast cell (MC) infiltration (A and B) and serum tryptase (C and D). r: Spearman’s correlation coefficient.
A
B
CD
Figure 3. Biomarkers of disease bur- den in indolent and advanced sys- temic mastocytosis (SM). Bone mar- row (BM) mast cell (MC) infiltration (A), serum tryptase levels (B), and KIT D816V mutant allele burden in BM aspirate/peripheral blood samples [variant allele frequency (VAF) liquid: C] and formalin-fixed paraffin-embed- ded (FFPE) BM sections (VAF tissue; D) were assessed for indolent (ISM) (blue, n=91) and advanced (green, n=25) SM patients. Samples from smoldering SM patients within the ISM cohort are shown in orange. **P<0.01; ***P<0.001.
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