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LEF-1 regulates β-catenin nuclear localization
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Figure 6. Modulation of LEF-1 expression affects relative nuclear β-catenin level. (A) Representative immunoblots showing the level and subcellular localization of total β-catenin and LEF-1 protein in K562 and HEL cells in response to control/LEF1 shRNA±CHIR99021 treatment. Shorter 6-hour (hrs) CHIR99021 treatments were used for these cells to minimize the LEF-1 expression induced through Wnt stimulation. (B) Summary graph showing the relative percentage nuclear β-catenin localization induced upon 6-hr CHIR99021 treatment of K562 or HEL cells±control/LEF-1 shRNA. (C) Effect of LEF1 shRNA on the proliferation of K562 and HEL at different concentrations of serum after 24, 48 and 72 hrs. (D) Representative immunoblots showing the level and subcellular localization of total β-catenin and LEF- 1 protein in ML-1 and U937 cells in response to control/LEF1 overexpression (o/e)± 16-hrs CHIR99021 treatment. The positions of full-length (FL) and short-forms (SF) of LEF-1 protein on the blot are indicated by arrows. Lamin A/C and a-tubulin were used to assess fraction purity and protein loading. (E) Summary graph showing the relative percentage nuclear β-catenin localization induced upon CHIR99021 treatment of ML-1 and U937 cells for 16 hrs ± control/LEF1 overexpression. *P<0.05; **P<0.01.
haematologica | 2019; 104(7)
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