Page 271 - Haematologica Vol. 109 - July 2024

LETTER TO THE EDITOR A
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Figure 1. Variant classification and heatmap representation of mutations detected at baseline in at least three patients with B-cell malignancies. Samples were sequenced to a median depth of >20,000 reads, with a validated sensitivity of 0.25% mutant allele frequency for all genomic regions, and 0.1% for mutational hotspots. Variant allele frequency (VAF) <0.1% for hotspot mutations and VAF <0.25% for non-hotspot mutations were excluded from the analysis. Germline and clonal hematopoiesis of indeterminate po- tential mutations were also excluded from the analysis. (A) The number of variants is shown on the X-axis. (B) DNA mutation profile of patients and the distribution of mutations among different study cohorts by mutation type and treatment status. Each column represents one patient, and each row represents one gene (indicated by the gene symbol on the left). Mutation rates of each gene are shown on the right. Mutation type is color-coded as shown in the figure. UTR: untranslated region; CLL: chronic lymphocytic leukemia; MCL: mantle cell lymphoma; MZL: marginal zone lymphoma; SLL: small lymphocytic lymphoma; WM: Waldenström mac- roglobulinemia.
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