Page 246 - Haematologica Vol. 109 - July 2024

ARTICLE - Application of CAAR T-cell therapy in ITP J. Zhou et al. A
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Figure 2. Generation of four anti-GPIbα hybridoma antibodies and validation of their binding epitopes with GPIbα chimeric autoanti- body receptors. (A) Binding detection of the 4 anti-GPIbα hybridoma antibodies (Gvb1/Gvb2/Gvb3/Gvb4) to human platelets using flow cytometry. Platelets were incubated with anti-human CD42b (GPIbα) antibody (clone: HIP1) and each anti-GPIbα hybridoma antibody (APC-conjugated) simultaneously. (B) B-cell receptor (BCR) density quantification for the 4 anti-GPIbα hybridoma cells. Hybridoma cells were stained with goat anti-mouse IgG antibodies (APC-conjugated). The mean fluorescence intensity (MFI) of BCR expression is shown in a histogram and bar graph. (C-F) Identification of the different binding epitopes of the hybridoma antibodies Gvb1, Gvb2, Gvb3, and
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