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L. Trentin et al.
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Figure 6. Reactive oxygen species (ROS) activity is associated with different cell cycle phases and cycling potential. (A) Low ROS activity in TTLshort/high proliferating (n=8) compared to TTLlong/slow proliferating (n=8) acute lymphoblastic leukemia samples: fold-change difference, (mean ± Standard Deviation) measured by flow cytometry. (B) Low ROS activity in sorted G1blow annotated primograft leukemia cells compared to G2/M cells measured by flow cytometry. TTLshort: Time To Leukemia short; TTLlong: Time To Leukemia long; ctrl: control.
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Figure 7. High leukemia initiating-cell activity in acute lymphoblastic leukemia (ALL) is associated with low reactive oxygen species (ROS) activity. (A) ALL cells with low ROS levels (ROSlow) are predominantly in early G0/G1 cell cycle phases, whereas cells with high ROS (ROShigh) include later S-G2/M phases. Simultaneous analysis of ROS activity and cell cycle distribution; cell cycle analysis in gated subpopulations of low or high (lower or upper 15%) ROS levels; one representative example of six analyses is shown. (B) Cell cycle distribution according to flow cytometry analysis in ROSlow and ROShigh subpopulations of 6 primograft ALL samples. (C) Increased engraftment activity of ROSlow ALL cells. Sorted ROShigh or ROSlow subfractions were transplanted (105 cells/mouse) and leukemia engraftment was analyzed as weeks from transplantation until appearance of ≥1% huCD19+ ALL cells in peripheral blood of the recipients. N=4 (ID03, ID04), n=6 (ID06) mice/group, log-rank test; P= statistical significance.
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haematologica | 2018; 103(6)